ProductCategory:Pre-maderecombinantAAV;AAV-Synapsin-FLPo;AAV-Syn-FLPo;AAV8-Synapsin-FLPo;AAV8-Syn-FLPo
Description: FLPoriginallyidentifiedfromSaccharomycescerevisiaeisasite-specificrecombinases(SSR)fromtheϜintegrasefamilyandrecognizesdistinct34bpFRTsequence.Ithasbeenwidelyusedforgeneratingconditionalknockoutmice,mediatedbyFLP/FRTsystem.InitialuseofFLPinmammaliancellsrevealedinefficientrecombinaseactivityduetoThermo-instABIlityoftheFLPprotein.Subsequentscreeningforthermo-stablemutantsresultedintheidentificationofFLPewhichwas4-foldincreaseinrecombinationefficiencyat37°C.HowevertherecombinationefficiencyofFLPeincellsremainsverylowbecauseofitsnon-mammalianorigin.
FLPoisamousecodonoptimizedFLPethatgreatlyincreasestheFRTrecombinationefficiencyforbothinvivoandinvitroinmouse. ThisAAV8-Synapsin-FLPopre-packagedrAAVinSEROtype8(withcapsidfromAAVserotype8and2xITRfromAAVserotype2)will over-expressFLPorecombinaseunderhumansynapsinpromoter(exclusiveneuronalexpression). Readytouseformat.
Titer:>1x1013VG/mL
Size:>30µL(StdPack)
StorageBuffer:PBS
InfectionProtocol:
VectorMap:
